Unveiling the fungal biotransformation of hydralazine using 13C-precursor. (August 2018)

• Record Type: Journal Article
• Authors: Almeida, Marilia O.; Lopes, Adriana A.; Roberto, Patricia G.; Bertoni, Bianca W.; Pupo, Mônica T.
• Is Part Of: Phytochemistry letters. Volume 26(2018)
• Page Start: 55
• Page End: 59
• Publication Date: 2018-08
• Subjects: Biotransformation -- Fusarium oxysporum SS46 -- Fusarium oxysporum ATCC MYA 4623 -- Hydralazine -- Epigenetic modifier; Botanical chemistry -- Periodicals; Chimie végétale -- Périodiques; 572.205
• DOI: 10.1016/j.phytol.2018.05.018
• Languages: English
• ISSNs: 1874-3900

Abstract

Graphical abstract: Highlights: Hydralazine is biotransformed in the same way by two Fusarium oxysporum strains. Feeding experiments using 1- 13 C-D-glucose and NMR established the biosynthesis. Hydralazine induced changes in fungal RNA expression profiles. Abstract: The fermentation of the endophyte Fusarium oxysporum SS46 and Fusarium oxysporum ATCC MYA 4623 with epigenetic modulator hydralazine in Czapek medium led to the isolation of 3-methyl-1, 2, 4-triazolo[3, 4 -a ]phthalazine (1 ). The mechanism of biotransformation involves reaction with acetyl-CoA and further cyclization, and was unveiled using 13 C-precursor. Additionally, AFLP technique for genomic DNA and differential gene expression (cDNA) analyses were applied for comparative profiling of the fungus under and without treatment with hydralazine. There was no change in the DNA profile of hydralazine-treated and non-treated cultures; however, cDNA-AFLP analyses showed a differential gene expression in the cultures after treatment of the fungi with hydralazine.