Artificial Control of Cell Signaling Using a Photocleavable Cobalt(III)–Nitrosyl Complex. (13th June 2019)

• Record Type: Journal Article
• Authors: Shin, Sangwon; Choe, Jisu; Park, Youngchan; Jeong, Donghyun; Song, Hyunjoon; You, Youngmin; Seo, Daeha; Cho, Jaeheung
• Is Part Of: Angewandte Chemie. Volume 131:Number 30(2019)
• Page Start: 10232
• Page End: 10237
• Publication Date: 2019-06-13
• Subjects: Cobalt(III)-Nitrosyl-Komplexe -- Kinase-Translokationsreporter -- Stickstoffmonoxid -- Photolyse -- Signaltransduktion; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201903106
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Cells use gaseous molecules such as nitric oxide (NO) to transmit both intracellular and intercellular signals. In principle, the endogenous small molecules regulate physiological changes, but it is unclear how randomly diffusive molecules trigger and discriminate signaling programs. Herein, it is shown that gasotransmitters use time‐dependent dynamics to discriminate the endogenous and exogenous inputs. For a real‐time stimulation of cell signaling, we synthesized a photo‐cleavable metal–nitrosyl complex, [Co III (MDAP)(NO)(CH3 CN)] 2+ (MDAP= N, N ′‐dimethyl‐2, 11‐diaza[3, 3](2, 6)pyridinophane), which can stably deliver and selectively release NO with fine temporal resolution in the cytosol, and used this to study the extracellular signal‐regulated kinases (ERKs), revealing how cells use both exogenous and endogenous NO to disentangle cellular responses. This technique can be to understand how diverse cellular signaling networks are dynamically interconnected and also to control drug delivery systems. Abstract : In neuem Licht : Ein Cobalt(III)‐Nitrosyl‐Komplex zur Untersuchung NO‐abhängiger Zellsignale wurde entwickelt und synthetisiert. Photolyse dieses Komplexes setzt NO mit hoher raumzeitlicher Kontrolle sowohl intra‐ als auch extrazellulär frei, was die Untersuchung von Zellsignalpfaden ermöglicht, die durch endogen und exogen produziertes NO aktiviert werden.