Enhancing peptaibols production in the biocontrol fungus Trichoderma longibrachiatum SMF2 by elimination of a putative glucose sensor. Issue 11 (2nd September 2019)

• Record Type: Journal Article
• Authors: Zhou, Yan‐Rong; Song, Xiao‐Yan; Li, Yue; Shi, Jin‐Chao; Shi, Wei‐Ling; Chen, Xiu‐Lan; Liu, Wei‐Feng; Liu, Xiang‐Mei; Zhang, Wei‐Xin; Zhang, Yu‐Zhong
• Is Part Of: Biotechnology and bioengineering. Volume 116:Issue 11(2019)
• Page Start: 3030
• Page End: 3040
• Publication Date: 2019-09-02
• Subjects: glucose sensor -- peptaibol -- secondary metabolite -- Trichoderma longibrachiatum; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.27138
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: Trichoderma spp. are main producers of peptide antibiotics known as peptaibols. While peptaibols have been shown to possess a range of biological activities, molecular understanding of the regulation of their production is largely unclear, which hampers the production improvement through genetic engineering. Here, we demonstrated that the orthologue of glucose sensors in the outstanding biocontrol fungus Trichoderma longibrachiatum SMF2, Tl STP1, participates in the regulation of peptaibols production. Deletion of Tlstp1 markedly impaired hyphal growth and conidiation, but significantly increased peptaibols yield by 5‐fold for Trichokonins A and 2.6‐fold for Trichokonins B. Quantitative real‐time polymerase chain reaction analyses showed that the increased peptaibols production occurs at the transcriptional levels of the two nonribosomal peptide synthetase encoding genes, tlx1 and tlx2 . Transcriptome analyses of the wild type and the Tlstp1 mutant strains indicated that Tl STP1 exerts a regulatory effect on a set of genes that are involved in a number of metabolic and cellular processes, including synthesis of several other secondary metabolites. These results suggest an important role of Tl STP1 in the regulation of vegetative growth and peptaibols production in T. longibrachiatum SMF2 and provide insights into construction of peptaibol‐hyperproducing strains through genetic engineering. Abstract : Trichoderma spp. are main producers of peptide antibiotics known as peptaibols. While peptaibols have been shown to possess a range of biological activities, molecular understanding of the regulation of their production is largely unclear, which hampers the production improvement through genetic engineering.