How pure are your vesicles?. Issue 1 (14th January 2013)

• Record Type: Journal Article
• Authors: Webber, Jason; Clayton, Aled
• Is Part Of: Journal of extracellular vesicles. Volume 2:Issue 1(2013)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2013-01-14
• Subjects: extracellular vesicles -- nano‐particle tracking -- vesicle counting -- sample purity; Cells -- Mechanical properties -- Periodicals; Transport Vesicles; Cells -- Mechanical properties; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; Periodicals; 571.63
• DOI: 10.3402/jev.v2i0.19861
• Languages: English
• ISSNs: 2001-3078

Abstract

Abstract : We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano‐vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA‐assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.