Artificial Control of Cell Signaling Using a Photocleavable Cobalt(III)–Nitrosyl Complex. Issue 30 (13th June 2019)

• Record Type: Journal Article
• Authors: Shin, Sangwon; Choe, Jisu; Park, Youngchan; Jeong, Donghyun; Song, Hyunjoon; You, Youngmin; Seo, Daeha; Cho, Jaeheung
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 30(2019)
• Page Start: 10126
• Page End: 10131
• Publication Date: 2019-06-13
• Subjects: cobalt(III)–nitrosyl complex -- kinase translocation reporter -- nitric oxide -- photolysis -- signal transduction; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201903106
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Cells use gaseous molecules such as nitric oxide (NO) to transmit both intracellular and intercellular signals. In principle, the endogenous small molecules regulate physiological changes, but it is unclear how randomly diffusive molecules trigger and discriminate signaling programs. Herein, it is shown that gasotransmitters use time‐dependent dynamics to discriminate the endogenous and exogenous inputs. For a real‐time stimulation of cell signaling, we synthesized a photo‐cleavable metal–nitrosyl complex, [Co III (MDAP)(NO)(CH3 CN)] 2+ (MDAP= N, N ′‐dimethyl‐2, 11‐diaza[3, 3](2, 6)pyridinophane), which can stably deliver and selectively release NO with fine temporal resolution in the cytosol, and used this to study the extracellular signal‐regulated kinases (ERKs), revealing how cells use both exogenous and endogenous NO to disentangle cellular responses. This technique can be to understand how diverse cellular signaling networks are dynamically interconnected and also to control drug delivery systems. Abstract : See things in a new light : A cobalt(III)–nitrosyl complex has been designed and synthesized for the study of NO‐dependent cell signaling. Photolysis of this complex releases NO with high spatiotemporal control, both intracellularly and extracellularly, allowing the study of the cell signaling pathways activated by endogenously and exogenously produced NO, respectively.