Laminin-521 promotes quiescence in isolated stellate cells from rat liver. (October 2018)

• Record Type: Journal Article
• Authors: Rohn, Friederike; Kordes, Claus; Castoldi, Mirco; Götze, Silke; Poschmann, Gereon; Stühler, Kai; Herebian, Diran; Benk, Amelie S.; Geiger, Fania; Zhang, Tingyu; Spatz, Joachim P.; Häussinger, Dieter
• Is Part Of: Biomaterials. Volume 180(2018)
• Page Start: 36
• Page End: 51
• Publication Date: 2018-10
• Subjects: Hepatic stellate cells -- Lama2 -- Lama5 -- Liver stem cell niche -- Basement membrane -- Space of dissé; 2AAF (2-acetylaminofluorene) -- α-Sma (α-smooth muscle actin) -- BSA (bovine serum albumin) -- Ck18 (cytokeratin 18) -- Ck19 (cytokeratin 19) -- Col1a2 (collagen type I α2 chain) -- Col4a1 (collagen type IV α1 chain) -- Dag1 (distroglycan-1) -- ECM (extracellular matrix) -- ESI (electrospray ionization) -- EV (extracellular vesicles) -- FCS (fetal calf serum) -- Fn (fibronectin) -- FS (functionalized surface) -- FS/LN-211 (functionalized surface with laminin-211) -- FS/LN-521 (functionalized surface with laminin-211) -- Gfap (glial fibrillary acidic protein) -- HRP (horseradish peroxidase) -- HCD (higher energy collisional dissociation) -- HSC (hepatic stellate cell) -- Itga3 (integrin α3) -- Itga6 (integrin α6) -- Itgb1 (integrin β1) -- Itgb4 (integrin β4) -- LAMA1 (laminin α1) -- LAMA2 (laminin α2) -- LAMA5 (laminin α5) -- LC-MS/MS (liquid chromatography-tandem mass spectrometry) -- LN-111 (laminin-111) -- LN-211 (laminin-211) -- LN-521 (laminin-521) -- Lrat (lecithin retinol acyltransferase) -- Lu/BCAM (Lutheran blood group/basal cell adhesion molecule) -- MRM (multiple reaction monitoring) -- MSC (mesenchymal stem cell) -- Nes (nestin) -- NTA (nanoparticle tracking analysis) -- PBS (phosphate buffered saline) -- PEG (poly(ethylene glycol)) -- PHx (partial hepatectomy) -- PS (polystyrene) -- PS/LN-211 (polystyrene coated with laminin-211) -- PS/LN-521 (polystyrene coated with laminin-211) -- Pparγ (peroxisome proliferator-activated receptor γ) -- qPCR (quantitative polymerase chain reaction) -- Reln (reelin) -- rcf (relative centrifugal force) -- Sdc1 (syndecan-1) -- SDS (sodium dodecyl sulfate) -- SEM (standard error of mean) -- Sparcl1 (secreted protein acidic and rich in cysteines-like 1) -- UHPLC (ultra-performance high pressure liquid chromatography); Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2018.07.008
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: The laminin α5 protein chain is an element of basement membranes and important to maintain stem cells. Hepatic stellate cells (HSC) are liver-resident mesenchymal stem cells, which reside in a quiescent state on a basement membrane-like structure in the space of Dissé. In the present study, laminin α5 chain was detected in the space of Dissé of normal rat liver. Since HSC are critical for liver regeneration and can contribute to fibrosis in chronic liver diseases, the effect of laminins on HSC maintenance was investigated. Therefore, isolated rat HSC were seeded on uncoated polystyrene (PS) or PS coated with either laminin-521 (PS/LN-521) or laminin-211 (PS/LN-211). PS/LN-521 improved HSC adhesion and better preserved their retinoid stores as well as quiescence- and stem cell-associated phenotype, whereas HSC on PS/LN-211 or PS developed into myofibroblasts-like cells. To improve the homogeneity as well as the presentation of laminin molecules on the culture surface to HSC, laminin-functionalized, gold-nanostructured glass surfaces were generated. This approach further enhanced the expression of quiescence-associated genes in HSC. In conclusion, the results indicate that LN-521 supports the quiescent state of HSC and laminin α5 can be regarded as an important element of their niche in the space of Dissé. Graphical abstract: Image 1 Highlights: Laminin-521 supports the quiescent state in hepatic stellate cells. Laminin-521-nanostructured surface improves hepatic stellate cell quiescence. Laminin α5 is detectable in liver sinusoids. Laminin α5 is upregulated during stem/progenitor cell-based liver regeneration. Laminin α5 is an important element of the stellate cell niche.