Carbon sequestration pathway of inorganic carbon in partial nitrification sludge. (December 2019)

• Record Type: Journal Article
• Authors: Liu, Xiaoning; Wang, Huaqin; Li, Haixiang; Jin, Yue; Zhang, Wenjie
• Is Part Of: Bioresource technology. Volume 293(2019)
• Publication Date: 2019-12
• Subjects: Ammonia-oxidizing bacteria -- Inorganic carbon -- 13C isotopic abundance -- Gene abundance; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122101
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Partial nitrification was conducted under different influent matrix concentrations. Carbon sequestration correlated positively with nitrogen removal (NH4 + -N substrate). Inorganic carbon concentration was negatively correlated with carbon sequestration. Functional genes for the Calvin cycle carbon sequestration pathway were present. The responsiveness of cbbL R1 gene abundance to NH4 + -N was high. Abstract: Inorganic carbon is an important carbon source of autotrophic bacteria, e.g., ammonia-oxidizing bacteria. Ammonia-oxidizing bacteria are chemoautotrophic bacteria with carbon sequestration capacity. Experiments were performed on partial nitrification sludge with different influent matrices, and optimal experimental operational conditions were established. The carbon fixation pathway of ammonia-oxidizing sludge was determined via 13 C isotope tracers and qPCR. The denitrification effect was better when the NH4 + -N, HCO3 –, Ca 2+, Mg 2+, and microbial accelerant concentrations were 15, 250, 113, 100 and 1 mL/L, respectively. The nitrite accumulation rate reached 96.95%. 13 C isotope tracing showed that 13 C abundance in sludge increased significantly. The results showed that IC added into the influent participated in the carbon metabolism of microorganisms. The functional gene cbbL, which follows the Calvin cycle carbon sequestration pathway, was identified in the ammonia-oxidizing bacteria, and the effect of influent NH4 + -N on the gene abundance was greater than that of other substrates.