Synthesis, characterization and cell imaging of a new polythiophene derivative. (November 2018)

• Record Type: Journal Article
• Authors: Wang, Fengyan; Wei, Junji; Xia, Huiyun; Yan, Ni; Song, Jiale; Zhang, Xiao; Gao, Lining; Song, Lifang; Yan, Luke
• Is Part Of: Dyes and pigments. Volume 158(2018)
• Page Start: 362
• Page End: 367
• Publication Date: 2018-11
• Subjects: Polythiophene derivative -- Cell imaging -- Lysosome-specific; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2018.05.070
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Fluorescent materials plays an essential role in the biological imaging field. Although numerous fluorescent materials have already been developed, new fluorescent materials with low cytotoxicity and good photostability are still urgently needed. In this work, a new polythiophene derivative PT-HMDA was designed and synthesized. PT-HMDA exhibits well water solubility, excellent photophysical properties, low cytotoxicity, and lysosome-specific cellular location. Thus, it could be a promising and instructive lysosome-specific cell imaging material with orange fluorescence emission. Highlights: A new polythiophene derivative PT-HMDA was designed and synthesized. The molar extinction coefficient, fluorescence quantum yield, and photostability of PT-HMDA were measured in water. The hydrodynamic diameter distribution of PT-HMDA were measured by dynamic light scattering (DLS) experiments. Cell viability assay demonstrated that PT-HMDA has low cytotoxicity to A549 cells. PT-HMDA could located in the cell lysosomes after co-location with lysosome-specific dye in A459 cells.