Teaching an Old Virus New Tricks: A Review on New Approaches to Study Age-Old Questions in Influenza Biology. Issue 21 (4th October 2019)

• Record Type: Journal Article
• Authors: Lakdawala, Seema S.; Lee, Nara; Brooke, Christopher B.
• Is Part Of: Journal of molecular biology. Volume 431:Issue 21(2019)
• Page Start: 4247
• Page End: 4258
• Publication Date: 2019-10-04
• Subjects: influenza virus -- microscopy -- RNA–protein interactions -- RNA–RNA interactions -- next-generation sequencing; vRNA viral RNA -- NGS next-generation sequencing -- FISH fluorescent in situ hybridization -- SPIM selective plane illumination microscope -- LOR limit of resolution -- EM electron microscopy -- NP nucleoprotein -- PacBio Pacific Biosciences -- ONT Oxford Nanopore Technologies -- DMS deep mutational scanning; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2019.04.038
• Languages: English
• ISSNs: 0022-2836

Abstract

Abstract: Influenza viruses have been studied for over 80 years, yet much about the basic viral lifecycle remain unknown. However, new imaging, biochemical, and sequencing techniques have revealed significant insight into many age-old questions of influenza virus biology. In this review, we will cover the role of imaging techniques to describe unique aspects of influenza virus assembly, biochemical techniques to study viral genomic organization, and next-generation sequencing to explore influenza genomic evolution. Our goal is to provide a brief overview of how emerging techniques are being used to answer basic questions about influenza viruses. This is not a comprehensive list of emerging techniques, rather ones that we feel will continue to make significant contributions to field of influenza biology. Graphical Abstract: Unlabelled Image Highlights: Many advanced technologies are used to answer age-old questions about influenza viruses. Microscopy approaches are used to study viral entry and assembly. Biochemical RNA biology techniques are used to study RNA–RNA and protein–RNA interactions. Next-generation sequencing tools help examine virus heterogeneity.