Activation of spinal macrophage-inducible C-type lectin induces mechanical allodynia and microglial activation in rats. (18th January 2019) - Yang, Jihoon
Highlights: Mincle is expressed in the dorsal and ventral horns of the lumbar spinal cord in rat. Mincle is present in neuron, but not microglia or astrocyte in the lumbar spinal cord. Intrathecal injection of Mincle ligand, trehalose dibehenate induces mechanical allodynia. Microglia is activated by intrathecal injection of Mincle ligand. Intrathecal minocycline reduces the allodynic response induced by Mincle activation. Abstract: Macrophage-inducible C-type lectin (Mincle), a pattern recognition receptor, is a critical component of the innate immune system that is involved in the pathogenesis of chronic pain. Previous studies have reported the expression of Mincle in neuronal and glial cells of the brain, but its expression and role in pain processing at the spinal level remain to be determined. The current study was performed to identify Mincle in the spinal cord and to investigate the effect of Mincle activation on spinal sensitization. Most Mincle immunoreactivity was localized within the grey matter and the dorsal and ventral horns of the lumbar spinal cord in naïve rats. A single intrathecal (i.t.) injection of trehalose-6, 6-dibehenate (TDB), a Mincle ligand, induced mechanical allodynia. Immunoreactivity to Mincle and Iba-1 in the spinal cord significantly increased after i.t. injection of TDB. Mechanical allodynia was attenuated by daily i.t. injection of minocycline. However, double immunofluorescence revealed that Mincle co-localizes with NeuN (neurons), but not with Iba-1 (microglia) or GFAP (astrocytes). In conclusion, we found that Mincle was present in spinal cord neurons, but not microglia or astrocytes, and may play a role in microglia-induced spinal sensitization.