Antioxidant peptides derived from the hydrolyzate of purple sea urchin (Strongylocentrotus nudus) gonad alleviate oxidative stress in Caenorhabditis elegans. (September 2018)

• Record Type: Journal Article
• Authors: Zhao, Sixu; Cheng, Qiong; Peng, Qiong; Yu, Xuesong; Yin, Xiquan; Liang, Ming; Ma, Chung Wah; Huang, Zebo; Jia, Weizhang
• Is Part Of: Journal of functional foods. Volume 48(2018)
• Page Start: 594
• Page End: 604
• Publication Date: 2018-09
• Subjects: Antioxidant peptide -- Oxidative stress -- Reactive oxygen species -- Purple sea urchin Strongylocentrotus nudus -- Caenorhabditis elegans; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.07.060
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: The hydrolyzate of sea urchin gonad exerts significant antioxidant activity. SnP7 (AAVPSGASTGIYEALELR) and SnP10 (NPLLEAFGNAK) could alleviate oxidative stress. Transcription factor DAF-16 is involved in SnP7- and SnP10-mediated antioxidant defenses. Antioxidant peptides could induce the expressions of DAF-16 target genes. Abstract: The papain hydrolyzate prepared from purple sea urchin ( Strongylocentrotus nudus ) gonad exerts significant antioxidant activity. Through ultrafiltration, gel filtration and high performance liquid chromatography, the active fractions from the hydrolyzate were screened by alleviating paraquat-induced oxidative stress in Caenorhabditis elegans, and the peptide components were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two antioxidant peptides SnP7 (AAVPSGASTGIYEALELR, 1805.03 Da) and SnP10 (NPLLEAFGNAK, 1173.34 Da) exhibit significant antioxidant capacity, which reduce reactive oxygen species (ROS) level and the expression of superoxide dismutase-3 (SOD-3) and heat shock protein-16.2 (HSP-16.2) in oxidation-damaged nematodes. Further studies indicated that SnP7 and SnP10 induce DAF-16 nuclear translocation and the expression of stress-related genes such as sod-3 . RNA interference (RNAi) suggested that peptide-induced enhancement of resistance is dependent on DAF-16. Taken together, S. nudus antioxidant peptides might act through activate DAF-16 signaling pathway, and induce the expression of DAF-16 target genes that enhance antioxidant potential of organisms.