Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study. (19th November 2012)

• Record Type: Journal Article
• Authors: Furuzawa-Carballeda, Janette; Lima, Guadalupe; Simancas, Perla; Ramos-Bello, Dolores; Simancas, Margaret; Bostock, Ian C.; Vilatobá, Mario; Gabilondo, Bernardo; Granados, Julio; Morales-Buenrostro, Luis; Alberú, Josefina; Llorente, Luis
• Other Names: Yamada Kazuhiko Academic Editor.
• Is Part Of: Journal of transplantation. Volume 2012(2012)
• Publication Date: 2012-11-19
• Subjects: Transplantation of organs, tissues, etc -- Periodicals; 617.95405
• DOI: 10.1155/2012/256960
• Languages: English
• ISSNs: 2090-0007

Abstract

Abstract : Regulatory Foxp3-expressing T cells (Tregs), IL-10-producing B cells (Bregs), and IDO-expressing dendritic cells (DCregs) downregulate inflammatory processes and induces peripheral tolerance. These subpopulations also might participate in maintaining allograft immunological quiescence in kidney transplant recipients (KTRs) with an excellent long-term graft function under immunosuppression (ELTGF). The aim of the study was to characterize and to enumerate peripheral Tregs, Bregs, and DCregs in KTR with an ELTGF for more than 5 years after transplant. Fourteen KTR with an ELTGF, 9 KTR with chronic graft dysfunction (CGD), and 12 healthy donors (HDs) were included in the study. CD19 + -expressing peripheral B lymphocytes were purified by positive selection. IL-10-producing B cells, CD4 + /CD25 hi, and CD8 + /CD28 − Tregs, as well as CCR6 + /CD123 + /IDO + DCs, were quantitated by flow cytometry. IL-10-producing Bregs (immature/transitional, but not CD19 + /CD38 hi /CD24 hi /CD27 + B10 cells), CCR6 + /CD123 + /IDO + DCs, and Tregs from ELTGF patients had similar or higher percentages versus HD (P < 0.05 ). By contrast, number of Tregs, DCregs, and Bregs except for CD27 + B10 cells from CGD patients had lower levels versus HD and ELTGF patients (P < 0.05 ). The findings of this exploratory study might suggest that in ELTGF patients, peripheral tolerance mechanisms could be directly involved in the maintenance of a quiescent immunologic state and graft function stability.