Influence of Estradiol-17beta on Progesterone and Estrogen Receptor mRNA Expression in Porcine Follicular Granulosa Cells during Short-Term, In Vitro Real-Time Cell Proliferation. (26th December 2016)

• Record Type: Journal Article
• Authors: Ciesiółka, Sylwia; Budna, Joanna; Jopek, Karol; Bryja, Artur; Kranc, Wiesława; Chachuła, Adrian; Borys, Sylwia; Dyszkiewicz Konwińska, Marta; Ziółkowska, Agnieszka; Antosik, Paweł; Bukowska, Dorota; Brüssow, Klaus P.; Bruska, Małgorzata; Nowicki, Michał; Zabel, Maciej; Kempisty, Bartosz
• Other Names: Jetten Anton M. Academic Editor.
• Is Part Of: BioMed research international. Volume 2016(2016)
• Publication Date: 2016-12-26
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2016/8431018
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Progesterone (P4) and estradiol (E2) play a significant role in mammalian reproduction. Our study demonstrated that separated porcine cumulus cells (CCs) and/or granulosa cells (GCs) might proliferate in vitro during short-term, real-time primary culture. The GCs were analyzed according to gene expression of the progesterone receptor (nuclear form) ( pgr ), progesterone receptor membrane component 1 ( pgrmc1 ), and estrogen-related receptor beta 3 ( esrrb3 ) in relation to two housekeeping genes: actb and pbgd . GCs were cultivated in medium with the E2. Both pgr / actb and pgr / pbgd revealed higher expression between 24 and 168 h of IVC of prolonged E2 treatment and at 48 h of IVC after acute E2 administration. The pgrmc1 / actb and pgrmc1 / pbgd displayed increased expression after prolonged E2 treatment between 24 and 120 h of IVC. The highest level of esrrb3 / actb at 120 and 144 h, as well as esrrb3 / pbgd at 120 h, in untreated controls as compared to the hormone-stimulated group, was observed. We suggest that E2 significantly influences the upregulation of pgr, pgrmc1, and esrrb3 expression in porcine GCs during real-time cell proliferation. Since esrrb3 expression is stimulated by E2 in both an acute and prolonged manner, estradiol may be recognized as a potential estrogen receptor agonist in GCs.