In vitro evaluation of 99mTc‐sultamicillin for infection imaging. (6th May 2021)

• Record Type: Journal Article
• Authors: Durkan, Kubra; Tuncel, Ayca; Yurt, Fatma
• Is Part Of: Biopharmaceutics & drug disposition. Volume 42:Number 6(2021)
• Page Start: 285
• Page End: 293
• Publication Date: 2021-05-06
• Subjects: bacterial binding -- in vitro -- infection -- sultamicillin -- technetium‐99m; Biopharmaceutics -- Periodicals; Drugs -- Metabolism -- Periodicals; Pharmacology -- Periodicals; Biopharmaceutics -- Periodicals; Pharmaceutical Preparations -- metabolism -- Periodicals; 615.19
• DOI: 10.1002/bdd.2281
• Languages: English
• ISSNs: 0142-2782

Abstract

Abstract: Early detection of the site of infection non‐invasively with radiolabeled molecules is important for the success of treatment. Technetium‐99m labeled antibiotics have the potential to discriminate between bacterial infection and sterile inflammation. Sultamicillin is the tosylate salt of the double ester of sulbactam plus ampicillin. In this study, sultamicillin was labeled with 99m Tc according to the stannous chloride method. Quality control studies of radiolabeled sultamicillin were performed by radiochromatographic methods. In vitro binding assays were performed in live and heat‐killed gram‐positive Staphylococcus aureus and gram‐negative Escherichia coli strains. The radiolabeling yield of 99m Tc‐sultamicillin was determined as 97.8% ± 3.1% (n = 5). The maximum bacterial uptake of 99m Tc‐sultamicillin was 80.7% ± 11.00% at 4 h for living S . aureus and 93.2% ± 4.40% at 2 h for E . coli . Bacterial uptake study results show that sultamicillin has the potential to be a nuclear imaging agent, especially in infections caused by gram‐negative E . coli and gram‐positive S . aureus . Abstract : Sultamicillin was labeled with 99m Tc according to the stannous chloride method with a high yield of 97.8 ± 3.1%. In vitro bacterial binding assays were performed in live and heat‐killed gram‐positive Staphylococcus aureus and gram‐negative Escherichia coli strains to determination of the potential to imaging infection. Sultamicillin has the potential to be a nuclear imaging agent in infections caused by Beta‐lactamresistant Gram‐negative E coli bacteria and gram‐positive S. aureus .