Leptin Promotes Prostate Cancer Proliferation and Migration by Stimulating STAT3 Pathway. (10th June 2021)

• Record Type: Journal Article
• Authors: Gorrab, Amal; Pagano, Alessandra; Ayed, Khouloud; Chebil, Mohamed; Derouiche, Amine; Kovacic, Hervé; Gati, Asma
• Is Part Of: Nutrition and cancer. Volume 73:Number 7(2021)
• Page Start: 1217
• Page End: 1227
• Publication Date: 2021-06-10
• Subjects: Cancer -- Periodicals; Nutrition -- Periodicals; Cancer; Nutrition; Periodicals; 616.9940654
• DOI: 10.1080/01635581.2020.1792946
• Languages: English
• ISSNs: 0163-5581

Abstract

Abstract: To better understand the link between obesity and prostate cancer (PC) aggressiveness, we investigate the role of leptin, an obesity associated adipokine, and its receptor (Ob-R) in PC cells migration. The migration assay (Wound-healing) was used to study the leptin impact on DU-145 and PC3 cells lines. STAT3 activation was performed by Western Blot. E-cadherin expression was studied using fluorescence microscopy and Ob-R expression in PC and benign prostatic Hyperplasia (BPH) biopsies was assessed by RT-PCR. In this study we demonstrate that high dose of leptin promotes PC cells migration and EMT transition via the stimulation of STAT3 pathway. In addition, we report that although Ob-R mRNA is expressed by ADK and BPH resections biopsies, significant higher levels were observed for ADK patients. Finally, we found a positive association between Ob-R mRNA expression and worse PC prognosis. A better understanding of the molecular processes of leptin signaling is crucial for identifying appropriate approaches for treatment of obesity-related PC patients.