Effects of pomegranate peel extract on ruminal and post-ruminal in vitro degradation of rumen inoculum of the dairy cow. Issue 3 (1st June 2021)

• Record Type: Journal Article
• Authors: Abarghuei, Mohammad Javad; Rouzbehan, Yousef; Salem, Abdelfattah Zeidan Mohamed; Zamiri, Mohammad Javad
• Is Part Of: Animal biotechnology. Volume 32:Issue 3(2021)
• Page Start: 366
• Page End: 374
• Publication Date: 2021-06-01
• Subjects: Dairy cow -- pomegranate-peel extract -- protein -- protozoa -- rumen; Animal biotechnology -- Periodicals; Animals, Domestic -- genetics -- Periodicals; Animals, Genetically Modified -- Periodicals; 660.6
• DOI: 10.1080/10495398.2020.1727492
• Languages: English
• ISSNs: 1049-5398

Abstract

Abstract: This experiment was carried out to study the effect of water extracted pomegranate peel extract (PE) on ruminal protein degradation and post-ruminal digestion in the dairy cow. PE was added at six levels of total phenolics (g/kg of the basal diet); 3.75 (PE1); 4.4 (PE2); 5.05 (PE3); 5.70 (PE4); and 6.35 (PE5). Rumen degradable crude protein (rdCP) decreased with PE addition ( L < 0.0001), but total CP degradability (tdCP) was not affected. Compared to PE0, PE2, and PE3 diets showed higher ( L = 0.054, Q = 0.029) digestibility of bypass CP (dBCP). Increasing levels of PE resulted in a decrease in proteolytic bacteria numbers ( p < 0.0001). At PE4 and PE5 levels, total VFA and acetate concentrations linearly decreased compared to PE0. PE inclusion lowered the acetate:propionate ratio ( L = 0.0001) and Ammonia-N production after 24 h ( L = 0.0008) of incubation. The total number of protozoa, genera Dasytricha and Isotricha, and subfamilies Entodiniinae, Diplodiniinae, and Ophrioscolecinae decreased with increasing dietary PE concentration ( p < 0.0001). The results suggest that all levels of PE addition reduce the protozoal population and Ammonia-N concentration. All PE levels slowed down protein degradation in the rumen but PE2 and PE3 showed the greatest effect.