In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis. (5th May 2011)

• Record Type: Journal Article
• Authors: Debunne, Manuelle; Portal, Christophe; Delest, Bruno; Brakenhielm, Ebba; Lallemand, Françoise; Henry, Jean-Paul; Ligeret, Heidi; Noack, Pauline; Massonneau, Marc; Romieu, Anthony; Renard, Pierre-Yves; Thuillez, Christian; Richard, Vincent
• Other Names: Jain D. Academic Editor.
• Is Part Of: International journal of molecular imaging. Volume 2011(2011)
• Publication Date: 2011-05-05
• Subjects: Molecular diagnosis -- Periodicals; Molecular probes -- Diagnostic use -- Periodicals; Diagnostic imaging -- Periodicals; Molecular Imaging; Diagnostic imaging; Molecular diagnosis; Electronic journals; Periodicals; Periodicals; 616.0757
• DOI: 10.1155/2011/413290
• Languages: English
• ISSNs: 2090-1712

Abstract

Abstract : Purpose . The aim of this paper is to develop new optical bioprobes for the imaging of apoptosis. Procedure . We developed quenched near-infrared probes which become fluorescent upon cleavage by caspase-3, the key regulatory enzyme of apoptosis. Results . Probes were shown to be selectively cleaved by recombinant caspase-3. Apoptosis of cultured endothelial cells was associated with an increased fluorescent signal for the cleaved probes, which colocalized with caspase-3 and was reduced by the addition of a caspase-3 inhibitor. Flow cytometry demonstrated a similar profile between the cleaved probes and annexin V. Ex vivo experiments showed that sections of hearts obtained from mice treated with the proapoptotic drug doxorubicin displayed an increase in the fluorescent signal for the cleaved probes, which was reduced by a caspase-3 inhibitor. Conclusion . We demonstrated the capacity of these novel probes to detect apoptosis by optical imaging in vitro and ex vivo .