A new anti-inflammatory flavonoid glycoside from tetraena aegyptia. Issue 12 (31st May 2021)

• Record Type: Journal Article
• Authors: Zaki, Ahmed A.; Xu, Xiaoqian; Wang, Yuewen; Shie, Pei-Hsin; Qiu, Longxin
• Is Part Of: Natural product research. Volume 35:Issue 12(2021)
• Page Start: 1985
• Page End: 1990
• Publication Date: 2021-05-31
• Subjects: Tetraena aegyptia -- Isorhamnetin -- RAW 264.7 -- Anti-inflammatory -- Cytokines; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2019.1650356
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: The chromatographic reinvestigation the methanol extract of Tetraena aegyptia led to the separation of a new flavonoid glycoside, isorhamnetin-3- O -[2```, 3```- O -isopropylidene-α-L-rhamnopyranosyl]-(1```→6``)- O -β-D-glucopyranoside (1 ), together with two known flavonoids, isorhamnetin (2 ) and isorhamnetin-3- O -glucoside (3 ), isolated for the first time from the plant. The new compound was evaluated for the anti-inflammatory activity by using LPS-induce RAW 264.7 cells model. Compound 1 showed significant inhibitory effect on NO release. ELISA assay showed a pronounced effect of 1 on the secretion of cytokines IL-6 and TNF-α, in a dose-dependent manner. Consistent results were obtained by qRT-PCR which revealed that compound 1 markedly reduced the mRNA expression of IL-6 and TNF-α. Together these data, we demonstrated the anti-inflammatory activity of compound 1 . Graphical Abstract: