Preparation of highly purified ω-3 docosapentaenoic acid from seal oil via urea complexation combined with preparative high performance liquid chromatography. Issue 10 (3rd July 2021)

• Record Type: Journal Article
• Authors: Mu, Hongyan; Li, Xiaodan; Jin, Qingzhe; Sun, Qingjie
• Is Part Of: Separation science and technology. Volume 56:Issue 10(2021)
• Page Start: 1769
• Page End: 1778
• Publication Date: 2021-07-03
• Subjects: Ω-3 docosapentaenoic acid (DPA) -- urea complexation -- optimization of the fractionation -- preparative high performance liquid chromatography (pre-HPLC); Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2020.1794895
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: Docosapentaenoic acid (DPA) derived from seal oil was concentrated by urea complexation and purified by preparative high performance liquid chromatography (pre-HPLC). A Box-Behnken Design (BBD) combined with response surface methodology (RSM) was applied to optimize urea complexation conditions. The maximum concentration of DPA (12.75%) with the yield of 48.54% were obtained at the FFA/Urea ratio of 1:3.7 (g/g) and crystallized at 1.3°C for 17.5 h. The concentrates were further fractionated by isocratic elution with the mixture of methanol and 0.1% aqueous phosphate on a C18 column, giving rise to a DPA purity to 93.12% with the yield of 38.37%. The present study could provide useful information and the highly purified products would contribute their beneficial effects for food and nutraceutical applications.