Multiresidue determination of 19 anabolic steroids in animal oil using enhanced matrix removal lipid cleanup and ultrahigh performance liquid chromatography-tandem mass spectrometry. Issue 21 (24th May 2021)

• Record Type: Journal Article
• Authors: Jiafeng, Yu; Decheng, Suo; Xiaoyong, Lei; Yang, Li; Guangyu, Lin; Min, Bi Su
• Is Part Of: Analytical methods. Volume 13:Issue 21(2021)
• Page Start: 2374
• Page End: 2383
• Publication Date: 2021-05-24
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d1ay00437a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A method for sensitive analysis of 19 anabolic steroids (AS) in animal oil using enhanced matrix removal lipid (EMR-Lipid) cleanup and ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. Abstract : A method for sensitive analysis of 19 anabolic steroids (AS) in animal oil using enhanced matrix removal lipid (EMR-Lipid) cleanup and ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. Oil samples were extracted with 20 mL of acetonitrile aqueous solution and purified using EMR-Lipid cartridges. The eluent was evaporated to dryness under nitrogen and analyzed by UHPLC-MS/MS using 0.1% formic acid–acetonitrile and 0.1% formic acid–water solutions as the mobile phase via gradient elution. The method effectively removed unwanted matrix co-extractives better than other extraction cleanup techniques while still delivering acceptable recovery results for most of the AS. The established quantification method showed AS recovery in the range of 72.9–110.7% with good precision (relative standard deviation < 15%).