Quaternary Ammonium Polyethyleneimine: Antibacterial Activity. (29th June 2010)

• Record Type: Journal Article
• Authors: Yudovin-Farber, Ira; Golenser, Jacob; Beyth, Nurit; Weiss, Ervin I.; Domb, Abraham J.
• Other Names: Fuchs Alan Academic Editor.
• Is Part Of: Journal of nanomaterials. Volume 2010(2010)
• Publication Date: 2010-06-29
• Subjects: Nanostructured materials -- Periodicals; Nanotechnology -- Periodicals; Nanomatériaux; Nanostructured materials; Nanotechnology; Nanostructures; Nanotechnology; Periodicals; Fulltext; Internet Resources; Periodicals; 620.115
• DOI: 10.1155/2010/826343
• Languages: English
• ISSNs: 1687-4110

Abstract

Abstract : Quaternary ammonium polyethyleneimine- (QA-PEI-) based nanoparticles were synthesized using two synthetic methods, reductive amination and N-alkylation. According to the first method, QA-PEI nanoparticles were synthesized by cross-linking with glutaraldehyde followed by reductive amination with octanal and further N-methylation with methyl iodide. The second method is based on crosslinking with dialkyl halide followed by N-alkylation with octyl halide and further N-methylation with methyl iodide. QA-PEI nanoparticles completely inhibited bacterial growth (>10 6 bacteria), including both Gram-positive, that is, Staphylococcus aureus at 80 μ g/mL, and Gram-negative, that is, Escherichia coli at 320 μ g/mL. Activity analysis revealed that the degree of alkylation and N-methylation of the QA-PEI nanoparticles plays a significant role in antibacterial activity of the reagent. The most potent compound was octyl alkylated QA-PEI alkylated at 1 : 1 mole ratio (primary amine of PEI monomer units/alkylating agent). Also, cytotoxicity studies on MAT-LyLu and MBT cell lines were performed with QA-PEI nanoparticles. These findings confirm previous reports that polycations bearing quaternary ammonium moieties inhibit bacterial growth in vitro and have a potential use as additives in medical devices which need antibacterial properties.