Sphingobacterium lumbrici sp. nov., a novel bacterium isolated from wormcast of Eisenia foetida. Issue 5 (28th May 2021)

• Record Type: Journal Article
• Authors: Zhang, Xiao-Fei; Shi, Rui; Chen, Min; Zhou, Xing-Kui; Wei, Yu-Qian; Cha, Qi-Yan; Li, Ni; Su, Yuan-Xiao; Ma, Li; Mo, Ming-He; Cao, Yi
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 5(2021)
• Publication Date: 2021-05-28
• Subjects: Sphingobacterium lumbrici sp. nov. -- 16S rRNA gene -- polyphasic taxonomic -- wormcast; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004823
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel Gram-stain-negative, rod-shaped, non-motile, yellowish bacterium, designated strain 1.3611 T, was isolated from the wormcast of Eisenia foetida . The strain grew optimally at 30–37 ℃, at pH 7.0 and with 0–1.0 % (w/v) NaCl. Based on the results of 16S rRNA gene sequence and phylogenetic analyses, strain 1.3611 T showed the highest degree of 16S rRNA gene sequence similarity to Sphingobacterium olei HAL-9 T (97.0 %), followed by Sphingobacterium alkalisoli Y3L14 T (95.8 %). The respiratory quinone of strain 1.3611 T was menaquinone-7 (MK-7) and its major cellular fatty acids were iso-C15 : 0 (41.3 %), summed feature 3 (C16 : 1 ω 7 c and/or C16 : 1 ω 6 c, 22.1 %) and iso-C17 : 0 3-OH (16.2 %). The major polar lipids were sphingophospholipid, phosphatidylethanolamine, four unidentified glycolipids, two unidentified phospholipids and five unidentified polar lipids. The genomic DNA G+C content was 39.0 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between the genomes of strain 1.3611 T and S. olei HAL-9 T were 37.9 and 88.9 %, respectively. According to the phenotypic and chemotaxonomic phylogenetic results, strain 1.3611 T should represent a novel species of the genus Sphingobacterium, for which the name Sphingobacterium lumbrici sp. nov. is proposed, with strain 1.3611 T (=KCTC 62980 T =CCTCC AB 2018349 T ) as the type strain.