Production, separation, and characterization of high-purity xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse. (March 2020)

• Record Type: Journal Article
• Authors: Li, Hailong; Chen, Xindong; Xiong, Lian; Zhang, Liquan; Chen, Xuefang; Wang, Can; Huang, Chao; Chen, Xinde
• Is Part Of: Bioresource technology. Volume 299(2020)
• Publication Date: 2020-03
• Subjects: Separation -- High-purity xylobiose -- Activated carbon adsorption -- Ethanol-water desorption; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122625
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: The adsorption and desorption process of crude XOS on activated carbon were studied. The impurities adsorbed on activated carbon can be selectively washed by water. The yield of xylobiose reached to 80.16% using 5-fold volume of 5% ethanol-water. Xylobiose with high-purity (97.29%) was characterized by HPLC, ESI-MS, and NMR. Abstract: The production of high-purity xylobiose from lignocellulose is an expensive and tedious process. In this work, the production of xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse was investigated. Furthermore, a simple process for the separation of xylobiose from enzymatic hydrolysate by activated carbon absorption, water washing, and ethanol-water desorption was developed. Under the optimized separation conditions, 96.77% xylobiose was adsorbed at 16% activated carbon loadings. Moreover, xylose and acetate could not be detected after washing by 3-fold volume of water. Xylobiose with 80.16% yield was eluted by 5-fold volume of 5% (v/v) ethanol-water. The reusability of activated carbon was evaluated by 5 cycles of adsorption-desorption process, suggesting that the activated carbon exhibited good reusability. The separated xylobiose sample with high-purity (97.29%) was confirmed by HPLC, ESI-MS, and NMR. Overall, this study provided a low-cost and robust technology for the production and separation of high-purity xylobiose from lignocellulose.