Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form. (11th December 2012)

• Record Type: Journal Article
• Authors: More, Snehal J.; Tandulwadkar, Suparna S.; Nikam, Ajinkya R.; Rathore, Atul S.; Sathiyanarayanan, L.; Mahadik, Kakasaheb R.
• Other Names: Ma J. Academic Editor.; Mitoma Y. Academic Editor.; Tena M. T. Academic Editor.
• Is Part Of: ISRN chromatography. Volume 2012(2012)
• Publication Date: 2012-12-11
• Subjects: Chromatographic analysis -- Periodicals; Chromatographic analysis; Electronic journals; Periodicals; Electronic journals; 543.8
• DOI: 10.5402/2012/252895
• Languages: English
• ISSNs: 2090-8636

Abstract

Abstract : A simple, precise, and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous determination of paracetamol (PCM), chlorzoxazone (CHZ), and nimesulide (NIM) in pharmaceutical dosage form. The chromatographic separation was achieved on a Thermo Hypersil GOLD C18 column (250 × 4.6 mm i.d., 5 μ m particle size). The mobile phase consisted of water : acetonitrile (55 : 45 v/v). The flow rate was set to 1.2 mL min −1 and UV detection was carried out at 275 nm. The retention time (t R ) for PCM, CHZ, and NIM was found to be 2.69 ± 0.02, 4.61 ± 0.01, and 9.55 ± 0.02 min, respectively. The validation of the proposed method was carried out for linearity, precision, robustness, limit of detection, limit of quantitation, specificity, and accuracy. The linear dynamic ranges were 32.5–65.0 μ g mL −1 for PCM, 37.5–75.0 μ g mL −1 for CHZ, and 10.0–20.0 μ g mL −1 for NIM. The developed method can be used for routine quality control analysis of titled drugs in pharmaceutical dosage form.