Colonization and growth of dehalorespiring biofilms on carbonaceous sorptive amendments. Issue 1 (2nd January 2019)

• Record Type: Journal Article
• Authors: Capozzi, Staci L.; Bodenreider, Coline; Prieto, Ana; Payne, Rayford B.; Sowers, Kevin R.; Kjellerup, Birthe Veno
• Is Part Of: Biofouling. Volume 35:Issue 1(2019)
• Page Start: 50
• Page End: 58
• Publication Date: 2019-01-02
• Subjects: Dehalorespiring biofilms -- activated carbon -- polychlorinated biphenyls (PCBs) -- quantitative confocal laser scanning microscopy (Q-CLSM) -- contaminated sediment; Marine fouling organisms -- Periodicals; Materials -- Biodeterioration -- Periodicals; Fouling -- Periodicals; Biodegradation -- Periodicals; Environmental Microbiology -- Periodicals; Water Microbiology -- Periodicals; 576.165
• DOI: 10.1080/08927014.2018.1563892
• Languages: English
• ISSNs: 0892-7014

Abstract

Abstract: Removal of polychlorinated biphenyls (PCBs) from contaminated sediments is a priority due to accumulation in the food chain. Recent success with reduction of PCB bioavailability due to adsorption onto activated carbon led to the recognition of in situ treatment as a remediation approach. In this study, reduced bioavailability and subsequent break-down of PCBs in dehalorespiring biofilms was investigated using Dehalobium chlorocoercia DF1. DF1 formed a patchy biofilm ranging in thickness from 3.9 to 6.7 µm (average 4.6 ± 0.87 µm), while the biofilm coverage varied from 5.5% (sand) to 20.2% (activated carbon), indicating a preference for sorptive materials. Quantification of DF1 biofilm bacteria showed 1.2–15.3 × 10 9 bacteria per gram of material. After 22 days, coal activated carbon, bone biochar, polyoxymethylene, and sand microcosms had dechlorinated 73%, 93%, 100%, and 83%, respectively. These results show that a biofilm-based inoculum for bioaugmentation of PCBs in sediment can be an efficient approach.