Mechanism of TangGanJian on nonalcoholic fatty liver disease with type 2 diabetes mellitus. (1st January 2018)

• Record Type: Journal Article
• Authors: Fan, Yanbo; Xiong, Wei; Li, Jingjing; Hu, Aimin; He, Zhiwei; Zhang, Jiawen; Zhou, Guoyun; Yin, Qiang
• Is Part Of: Pharmaceutical biology. Volume 56:Number 1(2018)
• Page Start: 567
• Page End: 572
• Publication Date: 2018-01-01
• Subjects: Therapy -- oxidative stress -- inflammation -- GSH-PX -- SOD -- TNF-α; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.1080/13880209.2018.1504972
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context: TangGanJian (TGJ) has a curative effect in the clinical treatment of nonalcoholic fatty liver disease (NAFLD) with type 2 diabetes mellitus (T2DM), while the mechanism involved in the treatment process remains unclear. Objective: This study details the mechanism of TGJ on the treatment of NAFLD with T2DM. Materials and methods: NAFLD was induced in T2DM rat model. Male Wistar rats were assigned into six groups: Group I (control), Group II (model), Group III (pioglitazone, 0.5 mg/kg), Group IV (high dose of TGJ, 24.8 g/kg), Group V (middle dose of TGJ, 12.4 g/kg) and Group VI (low dose of TGJ, 6.2 g/kg). All rats in each group were treated with the corresponding drugs by gavage for 8 weeks. Haematoxylin and eosin analysis was conducted. The indicators of inflammatory and oxidative stress were analysed utilizing one-way ANOVA. Results: The contents of TNF-α (15.794 ± 3.302 pg/mL), IL-6 (76.801 ± 8.491 pg/mL), IL-1β (100.101 ± 13.150 pg/mL), CRP (1.052 ± 0.079 pg/mL) and MDA (3.972 ± 0.159 pg/mL) were obviously elevated in NAFLD with T2DM rats compared to controls. Except for the IL-6, the levels of other markers declined in a dose-dependent manner after treatment with TGJ. The SOD (14.139 ± 1.479 U/mgprot) and GSH-PX (81.511 ± 5.276 U/mgprot) levels significantly decreased in NAFLD with T2DM rats, while the levels of these indicators increased after treatment with TGJ. Conclusions: TGJ may be a therapy for the NAFLD with T2DM rats by modulating the inflammatory response and the oxidative stress capacity.