Enhanced immunization techniques to obtain highly specific monoclonal antibodies. Issue 1 (2nd January 2018)

• Record Type: Journal Article
• Authors: de Almeida, Rodrigo; Nakamura, Cecília Naomi; de Lima Fontes, Marina; Deffune, Elenice; Felisbino, Sérgio Luis; Kaneno, Ramon; Fávaro, Wagner José; Billis, Athanase; Cerri, Marcel Otavio; Fusco-Almeida, Ana Marisa; Mendes Giannini, Maria José; Moroz, Andrei
• Is Part Of: MAbs. Volume 10:Issue 1(2018)
• Page Start: 46
• Page End: 54
• Publication Date: 2018-01-02
• Subjects: Cyclophosphamide -- high-zone tolerance -- immunogen -- monoclonal antibody -- multiple tolerization subtractive immunization -- neonatal tolerization -- subtractive immunization -- tolerogen; Monoclonal antibodies -- Therapeutic use -- Periodicals; Monoclonal antibodies -- Periodicals; Antibodies, Monoclonal -- Periodicals; 616.0798
• DOI: 10.1080/19420862.2017.1331804
• Languages: English
• ISSNs: 1942-0862

Abstract

ABSTRACT: Despite fast advances in genomics and proteomics, monoclonal antibodies (mAbs) are still a valuable tool for areas such as the evolution of basic research in stem cells and cancer, for immunophenotyping cell populations, diagnosing and prognosis of diseases, and for immunotherapy. To summarize different subtractive immunization approaches successfully used for the production of highly specific antibodies, we identified scientific articles in NCBI PubMed using the following search terms: subtractive immunization, monoclonal antibody, tolerization, neonatal, high-zone tolerance, masking immunization. Patent records were also consulted. From the list of results, we included all available reports, from 1985 to present, that used any enhanced immunization technique to produce either polyclonal or monoclonal antibodies. Our examination yielded direct evidence that these enhanced immunization techniques are efficient in obtaining specific antibodies to rare epitopes, with different applications, such as to identify food contaminants or tumor cells.