Ideal‐Filter Capillary Electrophoresis (IFCE) Facilitates the One‐Step Selection of Aptamers. (28th January 2019)

• Record Type: Journal Article
• Authors: Le, An T. H.; Krylova, Svetlana M.; Kanoatov, Mirzo; Desai, Shrey; Krylov, Sergey N.
• Is Part Of: Angewandte Chemie. Volume 131:Number 9(2019)
• Page Start: 2765
• Page End: 2769
• Publication Date: 2019-01-28
• Subjects: Aptamere -- DNA-Bibliotheken -- Oligonucleotide -- Einstufige Selektion -- Kapillarelektrophorese; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201812974
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Selection of aptamers from oligonucleotide libraries currently requires multiple rounds of alternating steps of partitioning of binders from nonbinders and enzymatic amplification of all collected oligonucleotides. Herein, we report a highly practical solution for reliable one‐step selection of aptamers. We introduce partitioning by ideal‐filter capillary electrophoresis (IFCE) in which binders and nonbinders move in the opposite directions. The efficiency of IFCE‐based partitioning reaches 10 9, which is ten million times higher than that of typical solid‐phase partitioning methods. One step of IFCE‐based partitioning is sufficient for the selection of a high‐affinity aptamer pool for a protein target. Partitioning by IFCE promises to become an indispensable tool for fast and robust selection of binders from different types of oligonucleotide libraries. Abstract : Eine gute Wahl : Die Idealfilter‐Kapillarelektrophorese (IFCE) wird für die zuverlässige und praktische, einstufige Selektion von Oligonucleotid‐Aptameren für Protein‐Zielstrukturen eingeführt. IFCE kann auch für die Selektion von Bindern aus DNA‐kodierten Bibliotheken niedermolekularer Verbindungen angewendet werden.