Rapid Quantification of Bacteria in Infected Root Canals Using Fluorescence Reagents and a Membrane Filter: A Pilot Study on Its Clinical Application to the Evaluation of the Outcomes of Endodontic Treatment. (27th May 2012)

• Record Type: Journal Article
• Authors: Sato, Takuichi; Yamaki, Keiko; Ishida, Naoko; Shoji, Megumi; Sato, Emika; Abiko, Yuki; Hashimoto, Kazuhiro; Takeuchi, Yasuhisa; Matsuyama, Junko; Shimauchi, Hidetoshi; Takahashi, Nobuhiro
• Other Names: Slutzky-Goldberg Iris Academic Editor.
• Is Part Of: International journal of dentistry. Volume 2012(2012)
• Publication Date: 2012-05-27
• Subjects: Dentistry -- Periodicals; Dentistry; Dentistry -- Periodicals; Electronic journals; Periodicals; 617.6
• DOI: 10.1155/2012/172935
• Languages: English
• ISSNs: 1687-8728

Abstract

Abstract : Objective . The bacterial examination has been performed during the course of the root canal treatment. In the present pilot study, the new developed method, using fluorescence reagents and a membrane filter, was applied to the detection and quantification of bacteria in infected root canals, in order to evaluate the outcomes of the treatment. Methods . Six infected root canals with periapical lesions from 5 subjects were included. Informed consent was obtained from all subjects (age ranges, 23–79 years). Samples from infected root canals were collected at the beginning of the treatment (termed #25 First), the end of the first day of treatment (termed #55 First), and the next appointment day (termed #55 Second). Then, the bacterial count (CFU) was measured using fluorescence reagents (4′, 6′-diamidino-2-phenylindole and propidium iodide) and the polycarbonate membrane filter by Bioplorer. Results . The mean ± SD of CFU in the sample of "#25 First" was ( 1.0 ± 1.4 ) × 10 5 . As the root canal treatment progressed, the CFU decreased as 7.9 × 10 3 (#55 First) and 4.3 × 10 2 (#55 Second). Conclusion . In the present pilot study, rapid detection and quantification of bacteria in infected root canals were found to be successfully performed using fluorescence reagents and a membrane filter (Bioplorer analysis).