ENHANCERS OF LIVER REGENERATION IN ONCOLOGICAL SURGERY. (18th May 2021)

• Record Type: Journal Article
• Authors: Osorio Capitan, M; Rose, S; Novo Sukia, I; Herrero de la Parte, B; Ruiz Montesinos, I; Garcia-Alonso, I
• Is Part Of: British journal of surgery. Volume 108(2021)Supplement 3
• Publication Date: 2021-05-18
• Subjects: Surgery -- Periodicals; 617.005
• DOI: 10.1093/bjs/znab160.010
• Languages: English
• ISSNs: 0007-1323

Abstract

Abstract: INTRODUCTION: Chemotherapy hinders liver function and probably its regenerating capacity, forcing to delay it after surgery. Our objective has been to verify this effect in an experimental model and to see if a hepatotrophic agent can prevent it. MATERIAL AND METHODS: Four groups of 6 WAG/RijHsd rats (males, 3-4 months) were submitted to ligation of the portal branch to the left lateral and left paramedian lobes. They were sacrificed 36 h later to quantify the percentage of liver corresponding to the ligated lobes (weight), the number of hepatocyte's nuclei (nº/100 µm 2 ) and their mean size (µm 2 ). One group received no treatment (control); another folic acid (2.5 mg/kg ip, during surgery); other 5-Fluorouracil (5-FU 50 mg/kg ip 48 h before); and the fourth received folic&5-FU. RESULTS: The animals treated with folic acid showed a greater number of hepatocyte's nuclei (24.4 ± 2.77 vs 15.2 ± 1.51) and their mean size was also greater (121 ± 2.34 vs 111 ± 1.8). However, the reduction in weight of the ligated parenchyma was less than in control group (33.4 ± 1.08 vs 29.5 ± 1.08). 5-FU did not modify the number of nuclei (15.6 ± 18.4), although they were smaller in size (104 ± 1.7). The addition of folic acid to 5-FU increased the number of nuclei (21.7 ± 2.8) and normalized their size (111 ± 3.2). CONCLUSIONS: 5-FU exerts a depressant effect on livers regeneration, and folic acid overcomes it. Thus, folic acid could allow early application of chemotherapy without affecting liver regeneration.