Dual Aptamer-Copper (II) Phosphate Nanocomposite-Based Point-of-Care Biosensor for the Determination of Escherichia coli O157:H7 through Pressure Monitoring with a Hand-Held Barometer. (10th September 2020)

• Record Type: Journal Article
• Authors: Zhao, Yinghao; Bu, Shengjun; Wang, Chengyu; Ma, Chengyou; Li, Zhongyi; Zhang, Wenhui; Wan, Jiayu
• Is Part Of: Analytical letters. Volume 54:Number 10(2021)
• Page Start: 1603
• Page End: 1615
• Publication Date: 2020-09-10
• Subjects: Aptamer -- copper (II) phosphate -- Escherichia coli O157:H7 -- hand-held barometer -- nanocomposites -- point-of-care biosensor; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2020.1817059
• Languages: English
• ISSNs: 0003-2719

Abstract

Abstract: Here, two types of aptamer (specific to E. coli O157:H7 pathogenic bacteria) Cu3 (PO4 )2 nanocomposites loaded with Fe3 O4 nanoparticles (aptamer@Fe3 O4, magnetic separation) and platinum nanoparticles (PtNPs) (aptamer@PtNP) were prepared in house via enzymatic hydrolysis of H2 O2 to O2 through a one-step method. In the proposed point-of-care biosensor, these nanocomposites were used as capture and signal probes for the determination of pathogenic E. coli O157:H7 bacteria via hand-held barometers detecting gas pressure signals in a sealed bottle. The pressure signal was linear when the concentration of E. coli O157:H7 was from 10 3 to 10 8 CFU/mL. With the easily prepared dual aptamer-Cu3 (PO4 )2 nanocomposites and the hand-held barometer signal readout, the proposed point-of-care biosensor was shown to be a potential tool for the determination of pathogenic bacteria in underdeveloped areas.