Toxicity of Silver Nanoparticles at the Air-Liquid Interface. (24th December 2012)

• Record Type: Journal Article
• Authors: Holder, Amara L.; Marr, Linsey C.
• Other Names: Alfaro-Moreno Ernesto Academic Editor.
• Is Part Of: BioMed research international. Volume 2013(2013)
• Publication Date: 2012-12-24
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2013/328934
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Silver nanoparticles are one of the most prevalent nanomaterials in consumer products. Some of these products are likely to be aerosolized, making silver nanoparticles a high priority for inhalation toxicity assessment. To study the inhalation toxicity of silver nanoparticles, we have exposed cultured lung cells to them at the air-liquid interface. Cells were exposed to suspensions of silver or nickel oxide (positive control) nanoparticles at concentrations of 2.6, 6.6, and 13.2 μ g cm −2 (volume concentrations of 10, 25, and 50 μ g ml −1 ) and to 0.7 μ g cm −2 silver or 2.1 μ g cm −2 nickel oxide aerosol at the air-liquid interface. Unlike a number of in vitro studies employing suspensions of silver nanoparticles, which have shown strong toxic effects, both suspensions and aerosolized nanoparticles caused negligible cytotoxicity and only a mild inflammatory response, in agreement with animal exposures. Additionally, we have developed a novel method using a differential mobility analyzer to select aerosolized nanoparticles of a single diameter to assess the size-dependent toxicity of silver nanoparticles.