Human Acquired Aplastic Anemia Patients' Bone-Marrow-Derived Mesenchymal Stem Cells Are Not Influenced by Hematopoietic Compartment and Maintain Stemness and Immune Properties. (30th April 2021)

• Record Type: Journal Article
• Authors: Sharma, Vandana; Rawat, Sonali; Gupta, Suchi; Tamta, Sweta; Sharma, Rinkey; Seth, Tulika; Mohanty, Sujata
• Other Names: Hassen Kalkidan Academic Editor.
• Is Part Of: Anemia. Volume 2021(2021)
• Publication Date: 2021-04-30
• Subjects: Anemia -- Periodicals; 616.152005
• DOI: 10.1155/2021/6678067
• Languages: English
• ISSNs: 2090-1267

Abstract

Abstract : Background and Objective . Acquired aplastic anemia (aAA) is a bone marrow failure disorder characterized by pancytopenia and bone marrow aplasia. Bone marrow Mesenchymal Stem Cells (BM-MSCs) are an important component of BM microenvironment, associated with hematopoietic and immune homeostasis. Any alterations in BM microenvironment can disrupt the normal functioning and it needs to be assessed. Methods . In the current study, we investigated the morphological differences, proliferation capacity, population doubling time (PDT), surface marker profiling, trilineage differentiation potential, and immunosuppressive ability of BM Mesenchymal Stem Cells (BM-MSCs) from untreated aAA patients and in the same number of age- and gender-matched controls. Results . We observed similar morphology, proliferation capacity, phenotype, trilineage differentiation potential, and immunomodulatory properties of BM-MSCs in aAA patients and control subjects. Conclusion . Our results confirm that the basic and immunosuppressive properties of BM-MSCs from aAA patients do not differ from normal BM-MSCs. Our data suggest that BM-MSCs from aAA patients might not be involved in disease pathogenesis. However, owing to a smaller number of samples, it is not conclusive, and future studies with more exhaustive investigation at transcriptome level are warranted.