Display of the Viral Epitopes on Lactococcus lactis: A Model for Food Grade Vaccine against EV71. (13th February 2013)

• Record Type: Journal Article
• Authors: Varma, Nadimpalli Ravi S.; Toosa, Haryanti; Foo, Hooi Ling; Alitheen, Noorjahan Banu Mohamed; Nor Shamsudin, Mariana; Arbab, Ali S.; Yusoff, Khatijah; Abdul Rahim, Raha
• Other Names: Wei Yu Hong Academic Editor.
• Is Part Of: Biotechnology research international. Volume 2013(2013)
• Publication Date: 2013-02-13
• Subjects: Biotechnology -- Periodicals; Biotechnology; Biotechnology; Periodicals; 660.6
• DOI: 10.1155/2013/431315
• Languages: English
• ISSNs: 2090-3138

Abstract

Abstract : In this study, we have developed a system for display of antigens of Enterovirus type 71 (EV71) on the cell surface of L. lactis . The viral capsid protein (VP1) gene from a local viral isolate was utilized as the candidate vaccine for the development of oral live vaccines against EV71 using L. lactis as a carrier. We expressed fusion proteins in E. coli and purified fusion proteins were incubated with L. lactis . We confirmed that mice orally fed with L. lactis displaying these fusion proteins on its surface were able to mount an immune response against the epitopes of EV71. This is the first example of an EV71 antigen displayed on the surface of a food grade organism and opens a new perspective for alternative vaccine strategies against the EV71. We believe that the method of protein docking utilized in this study will allow for more flexible presentations of short peptides and proteins on the surface of L. lactis to be useful as a delivery vehicle.