A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device. (14th September 2013)

• Record Type: Journal Article
• Authors: Zhang, Chen; Jang, Sunyoung; Amadi, Ovid C.; Shimizu, Koichi; Lee, Richard T.; Mitchell, Richard N.
• Other Names: Wada Koichiro Academic Editor.
• Is Part Of: BioMed research international. Volume 2013(2013)
• Publication Date: 2013-09-14
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2013/373569
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Existing chemotaxis assays do not generate stable chemotactic gradients and thus—over time—functionally measure only nonspecific random motion ( chemokinesis ). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis. We describe here a novel microfluidic device for sensitive assay of cellular migration and show its application for evaluating the chemotaxis of smooth muscle cells in a chemokine gradient.