Hypnotic Effect of A. absinthium Hydroalcoholic Extract in Pentobarbital-Treated Mice. (22nd April 2021)

• Record Type: Journal Article
• Authors: Rakhshandeh, Hassan; Heidari, Amirhossein; Pourbagher-Shahri, Ali Mohammad; Rashidi, Roghayeh; Forouzanfar, Fatemeh
• Other Names: de Carvalho Mamede Academic Editor.
• Is Part Of: Neurology research international. Volume 2021(2021)
• Publication Date: 2021-04-22
• Subjects: Neurology -- Periodicals; Nervous system -- Diseases -- Periodicals; 616.8005
• DOI: 10.1155/2021/5521019
• Languages: English
• ISSNs: 2090-1852

Abstract

Abstract : Background . Current drugs used in the management of insomnia are associated with side effects. The use of medicinal herbs for insomnia treatment has recently been suggested. Objective . The present study aimed to determine the hypnotic activity of the hydroalcoholic extract of Artemisia absinthium (A . absinthium) in mice. Method . The toxicity of A . absinthium extract is assessed by their lethal dose 50% (LD50), and cytotoxicity evaluation was also done with PC12 cell lines by MTT assay. A . absinthium extract (25, 50, 100, and 200 mg/kg) and 3 fractions ( n -butanol fraction (NBF), ethyl acetate fraction (EAF), and aqueous fraction (AQF)) were administered intraperitoneally30 minutes before 30 mg/kg pentobarbital intraperitoneal injection; after that, the sleeping time and sleep latency were recorded. Results . The LD50 value was 2.4 g/kg. The extracts tested showed no negative effect on the proliferation of PC12 cells. A . absinthium extract increased the duration of pentobarbital-induced sleep at doses of 100 and 200 mg/kg (P < 0.01 -P < 0.001 ). Similarly, AQF, EAF, and NBF at 200 mg/kg could increase sleep duration (P < 0.05 ). The sleep latency was decreased by A . absinthium extract at doses of 100 and 200 mg/kg (P < 0.05 -P < 0.01 ), AQF (P < 0.05 ), and EAF ( P < 0.05). Besides, flumazenil reversed the hypnotic effect of A . absinthium extract (P < 0.05 ). Conclusion . A . absinthium extract probably demonstrated sleep-enhancing effects by regulating GABAergic system.