N3‐Kethoxal‐Based Bioorthogonal Intracellular RNA Labeling. (5th February 2021)

• Record Type: Journal Article
• Authors: Huang, Jinguo; Zhao, Ruiqi; Mo, Jing; Wang, Fang; Weng, Xiaocheng; Zhou, Xiang
• Is Part Of: Chembiochem. Volume 22:Number 9(2021)
• Page Start: 1559
• Page End: 1562
• Publication Date: 2021-02-05
• Subjects: azides -- bioorthogonal -- intracellular -- probes -- RNA; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202000755
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: There is growing interest in developing intracellular RNA tools. Herein, we describe a strategy for N3 ‐kethoxal (N3 K)‐based bioorthogonal intracellular RNA functionalization. With N3 K labeling followed by an in vivo click reaction with DBCO derivatives, RNA can be modified with fluorescent or phenol groups. This strategy provides a new way of labeling RNA inside cells. Abstract : The small, membrane‐permeable probe N3 ‐kethoxal has low toxicity and highly selectivity for reaction with guanine allowing it to introduce azido groups into RNA within a cellular environment. This N3 K‐assisted bioorthogonal intracellular RNA strategy provides a great prospect for in vivo RNA labeling to develop various RNA tools for RNA functional research.