Evaluating the influence of progesterone concentration and time of exposure on in vitro endometrial decidualisation. (15th May 2021)

• Record Type: Journal Article
• Authors: Suthaporn, Sutham; Jayaprakasan, Kanna; Thornton, Jim G.; Walker, Kate F.; Castellanos, Marcos; May, Sean; Hernandez-Medrano, Juan; Maalouf, Walid E.
• Is Part Of: Molecular and cellular endocrinology. Volume 528(2021)
• Publication Date: 2021-05-15
• Subjects: Progesterone -- Endometrium -- Implantation window -- Decidualisation -- Human endometrial stromal cells -- Microarray; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2021.111242
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: This study aimed to evaluate the influence of progesterone (concentration and time of exposure) on endometrial decidualisation using an in vitro model cell line: Human Endometrial Stromal Cells (HESCs). HESCs exposed to progesterone (1 and 10 μM) had higher percentages of decidualised cells and higher expression of the decidual marker (Insulin Like Growth Factor Binding Protein 1 (IGFBP1)) compared with those exposed to (0.1 μM). Among those HESCs cultured with 1 μM progesterone for 11 days, the highest rate of morphological differentiation (40–50%) occurred between days 7–9 and IGFBP1 peaked on day 7. The cell-cycle pathway was significantly down-regulated in HESCs exposed to at least 1 μM progesterone regardless of the incubation period. We conclude that exposure to high progesterone concentration for 7–9 days is essential to maximise the process of decidualisation. Highlights: A high progesterone concentration is associated with improvement in the decidualisation process. The most functionally and morphologically active part of the decidualisation occurred 7–9 days after progesterone exposure. Progesterone promotes downregulation of the cell-cycle pathway and upregulation of genes-related to decidualisation.