A switch of microbial flora coupled with ontogenetic niche shift in Leptinotarsa decemlineata. Issue 1 (16th March 2021)

• Record Type: Journal Article
• Authors: Kang, Wei‐Nan; Jin, Lin; Fu, Kai‐Yun; Guo, Wen‐Chao; Li, Guo‐Qing
• Is Part Of: Archives of insect biochemistry and physiology. Volume 107:Issue 1(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-03-16
• Subjects: antibiotics -- bacteria diversity -- fitness -- Leptinotarsa decemlineata -- ontogenetic niche shift; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21782
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: In Leptinotarsa decemlineata, a final‐instar wandering larva typically undergoes an ontogenetic niche shift (ONS), from potato plant during the foraging stage to its pupation site below ground. Using high‐throughput sequencing of the bacterial 16S ribosomal RNA gene, we determined the hypothesis that the L. decemlineata pupae harbor stage‐specific bacteria to meet the physiological requirements for underground habitat. We identified 34 bacterial phyla, comprising 73 classes, 208 orders, 375 families, and 766 genera in the collected specimens. Microbes across phyla Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes were enriched in the pupae, while those in the phylum Proteobacteria, Tenericutes, Firmicutes, and Bacteroidetes dominated in the larvae and adults. A total of 18 genera, including Blastococcus, Corynebacterium _1, Gordonia, Microbacterium, Nocardia, Nocardioides, Rhodococcus, Solirubrobacter, Tsukamurella, Enterococcus, Acinetobacter, Escherichia_Shigella, Lysobacter, Pseudomonas, and Stenotrophomonas, were specifically distributed in pupae. Moreover, soil sterilizing removed a major portion of bacteria in pupae. Specifically, both Enterococcus and Pseudomonas were eliminated in the soil sterilizing and antibiotic‐fed beetle groups. Furthermore, the pupation rate and fresh pupal weight were similar, whereas the emergence rate and adult weight were decreased in the antibiotic‐fed beetles, compared with controls. The results demonstrate that a switch of bacterial communities occurs in the pupae; the pupal‐specific bacteria genera are mainly originated from soil; this bacterial biodiversity improves pupa performance in soil. Our results provide new insight into the evolutionary fitness of L. decemlineata to different environmental niches. Abstract : Using high‐throughput sequencing of the bacterial 16S ribosomal RNA gene, we find that a switch of bacterial communities occurs in the pupae. A comparison of control and sterilized soil reveals the pupal‐specific bacteria genera are mainly originated from soil. This bacterial biodiversity improves pupa performance in soil. When removed these bacteria, the emergence rate and adult weight is decreased. Research Highlights: A switch of microbial flora was coupled with ontogenetic niche shift. A total of 18 genera were specifically distributed in pupae. Bacteria removal decreased adult weight and declined emergence rate.