Racemases and epimerases operating through a 1, 1-proton transfer mechanism: reactivity, mechanism and inhibition. (12th April 2021)

• Record Type: Journal Article
• Authors: Lloyd, Matthew D.; Yevglevskis, Maksims; Nathubhai, Amit; James, Tony D.; Threadgill, Michael D.; Woodman, Timothy J.
• Is Part Of: Chemical Society reviews. Volume 50:Number 10(2021)
• Page Start: 5952
• Page End: 5984
• Publication Date: 2021-04-12
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cs00540a
• Languages: English
• ISSNs: 0306-0012

Abstract

Abstract : Racemases and epimerases using a deprotonation/reprotonation mechanism are important drug targets and have important biotechnological applications. This review focuses on the reactivity, mechanism, and inhibition of these versatile enzymes. Abstract : Racemases and epimerases catalyse changes in the stereochemical configurations of chiral centres and are of interest as model enzymes and as biotechnological tools. They also occupy pivotal positions within metabolic pathways and, hence, many of them are important drug targets. This review summarises the catalytic mechanisms of PLP-dependent, enolase family and cofactor-independent racemases and epimerases operating by a deprotonation/reprotonation (1, 1-proton transfer) mechanism and methods for measuring their catalytic activity. Strategies for inhibiting these enzymes are reviewed, as are specific examples of inhibitors. Rational design of inhibitors based on substrates has been extensively explored but there is considerable scope for development of transition-state mimics and covalent inhibitors and for the identification of inhibitors by high-throughput, fragment and virtual screening approaches. The increasing availability of enzyme structures obtained using X-ray crystallography will facilitate development of inhibitors by rational design and fragment screening, whilst protein models will facilitate development of transition-state mimics.