In vitro antioxidant and wound-healing activities of hydrolyzed collagen from defatted Asian sea bass skin as influenced by different enzyme types and hydrolysis processes. Issue 30 (19th May 2021)

• Record Type: Journal Article
• Authors: Chotphruethipong, Lalita; Binlateh, Thunwa; Hutamekalin, Pilaiwanwadee; Sukketsiri, Wanida; Aluko, Rotimi E.; Benjakul, Soottawat
• Is Part Of: RSC advances. Volume 11:Issue 30(2021)
• Page Start: 18144
• Page End: 18151
• Publication Date: 2021-05-19
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra03131g
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Hydrolyzed collagen (HC) from sea bass skin prepared using papain and Alcalase had antioxidant potency and could enhance MRC-5 cell proliferation and lamellipodia formation. HC can be used as a nutraceutical or functional food ingredient. Abstract : Hydrolyzed collagen (HC) from defatted Asian sea bass skin was prepared by different enzymatic hydrolysis processes. For one-enzyme hydrolysis, papain (0.3 unit per g dry matter, DM) at 40 °C for 90 min or Alcalase (0.2 or 0.3 unit per g DM) at 50 °C for 90 min were used. The two-enzyme hydrolysis was accomplished with papain at 0.3 unit per g DM (P0.3), followed by Alcalase hydrolysis at 0.2 or 0.3 units per g DM (A0.2 or A0.3, respectively). HC prepared using the P0.3 + A0.3 process showed higher peptide yield, recovery and imino acid content in addition to stronger ABTS, DPPH radical scavenging activities and ferric reducing antioxidant power than other hydrolysis processes. HC obtained from the P0.3 + A0.3 process (at 125–500 μg mL −1 ) induced MRC-5 fibroblast proliferation and augmented migration and lamellipodia formation in the cells. Peptides with average molecular weight of 750 Da exhibited the highest ABTS radical scavenging activity while the 4652 Da fraction had the lowest. Thus, HC can be considered as a suitable ingredient to formulate functional products for skin nourishment and wound healing.