Reconstitution of Abscisic Acid Signaling from the Receptor to DNA via bHLH Transcription Factors  . Issue 2 (24th April 2017)

• Record Type: Journal Article
• Authors: Takahashi, Yohei; Ebisu, Yuta; Shimazaki, Ken-ichiro
• Is Part Of: Plant physiology. Volume 174:Issue 2(2017)
• Page Start: 815
• Page End: 822
• Publication Date: 2017-04-24
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.16.01825
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : Signaling from ABA receptor to its target DNA is reconstituted using basic helix-loop-helix (bHLH) transcriptional activator AKS1 in vitro. Abstract: The plant hormone abscisic acid (ABA ) confers drought tolerance in plants through stomatal closure and regulation of gene expression. The complex consisting of the ABA receptor PYRABACTIN RESISTANCE/REGULATORY COMPONENTS OF ABA RECEPTOR (PYR/RCAR), type 2C protein phosphatase (PP2C), and SNF1-related protein kinase 2 (SnRK2) has a key role in ABA signaling. Basic helix-loop-helix (bHLH) transcriptional activator ABA-RESPONSIVE KINASE SUBSTRATE1 (AKS1, also known as FBH3) is released from DNA by phosphorylation-induced monomerization in response to ABA in guard cells. Here we reconstituted the release of AKS1 from DNA via the ABA signaling core complex in vitro. We first obtained evidence to confirm that AKS1 is an endogenous substrate for SnRK2s. Phosphorylation of AKS1 and activation of SnRK2 showed the same time course in response to ABA in guard cells. AKS1 was bound to SnRK2.6 in vivo. Three ABA -responsive SnRK2s (SnRK2.2/SRK2D, SnRK2.3/SRK2I, and SnRK2.6/SRK2E/OST1) phosphorylated AKS1 in vitro, and the phosphorylation was eliminated by the triple mutation of SnRK2s in plants. We reconstituted the AKS1 phosphorylation in vitro via the signaling complex containing the ABA receptor PYR1, a PP2C, HYPERSENSITIVE TO ABA1 (HAB1), and a protein kinase, SnRK2.6 in response to ABA . We further reconstituted the release of AKS1 from the target gene of POTASSIUM CHANNEL IN ARABIDOPSIS THALIANA 1 ( KAT1 ) via the complex in response to ABA . These results demonstrate that AKS1 provides a link between the signaling complex and ABA -responsive genes and furnish evidence for a minimal signaling mechanism from ABA perception to DNA.