Comparison of the in vitro assays to investigate the hepatic metabolism of seven pesticides in Cyprinus carpio and Oncorhynchus mykiss. (August 2021)

• Record Type: Journal Article
• Authors: Tust, Maurice; Kohler, Maxie; Lagojda, Andreas; Lamshoeft, Marc
• Is Part Of: Chemosphere. Volume 277(2021)
• Publication Date: 2021-08
• Subjects: Metabolism -- Fish hepatocytes -- Liver S9 fraction -- Common carp -- Rainbow trout -- Bluegill sunfish -- Pesticides; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.130254
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Liver S9 fractions from common carp ( Cyprinus carpio ) and rainbow trout ( Oncorhynchus mykiss) were incubated with seven pesticides (fenamidone, fenoxaprop-p-ethyl, penflufen, spirotetramat, tebuconazole, tembotrione and trifloxystrobin) and the metabolic pathways of the applied chemicals were determined by HPLC-high-resolution mass spectrometry. Five of the seven pesticides (fenamidone, penflufen, spirotetramat, trifloxystrobin and fenoxaprop-p-ethyl) revealed a higher metabolic capacity of rainbow trout liver fractions compared to carp liver fractions. The other two pesticides (tebuconazole and tembotrione) showed a similar and marginal biotransformation for liver S9 fractions of both species. Furthermore, four compounds (penflufen, spirotetramat, tembotrione and tebuconazole) were incubated with cryo-preserved hepatocytes of rainbow trout showing additional conjugated metabolites compared to liver S9 fractions. The incubations were performed with concentrations of 1 and 10 μM for experiments with liver S9 fractions and 5 μM with hepatocytes for up to 120 (liver S9 fractions) or 240 min (hepatocytes). A set of positive controls was used to confirm the metabolic capability of the in vitro systems. The comparison of the in vitro results from hepatocyte assays of penflufen and tebuconazole with the data from corresponding in vivo studies performed according to OECD (Organisation for Economic Co-operation and Development) guideline 305 exhibited a similar metabolic behavior for these pesticides and emphasizes the reliability of the in vitro assays. Besides investigation of the metabolism of plant protection products for research purposes, inter-species comparison by in vitro assays and the use of PBTK modelling approaches will allow improved environmental and dietary risk assessments. Graphical abstract: Image 1 Highlights: Evaluation of in vitro liver assays of Cyprinus carpio and Oncorhynchus mykiss . Metabolic investigation of seven pesticides in liver S9 fractions and hepatocytes. Rainbow trout metabolized faster than common carp for most tested pesticides. High similarity of in vitro and in vivo metabolic pathways.