Reduction of pollution load of tannery effluent by cell immobilization approach using Ochrobactrum intermedium. (June 2021)

• Record Type: Journal Article
• Authors: Yadav, Pooja; Yadav, Ashutosh; Srivastava, Janmejai Kumar; Raj, Abhay
• Is Part Of: Journal of water process engineering. Volume 41(2021)
• Publication Date: 2021-06
• Subjects: Tannery effluent -- O. intermedium strain IITR002 -- PVA bead -- Pollutant load reduction -- Phytotoxicity; Water-supply engineering -- Periodicals; Saline water conversion -- Periodicals; Seawater -- Distillation -- Periodicals; Sanitary engineering -- Periodicals; Sewage -- Purification -- Periodicals; 627
• DOI: 10.1016/j.jwpe.2021.102059
• Languages: English
• ISSNs: 2214-7144

Abstract

Graphical abstract: Highlights: A chromium tolerant Ochrobactrum intermedium strain IITR002 was isolated. Pollution load of tannery effluent reduced significantly after treatment with bacterial immobilized polyvinyl alcohol beads. GC–MS analysis revealed degradation or transformation of native complex compounds by Ochrobactrum intermedium. Bacterial treatment of tannery effluents reduced phytotoxic effects. Abstract: The purpose of this present study was to reduce the pollution load of the tannery effluent through a cell immobilization approach using chromium tolerant bacteria isolated from tannery sludge. Among various initially isolated bacteria, strain IITR002 exhibited the highest pollution load reduction was characterized as Ochrobactrum intermedium by 16S rDNA sequencing. The bacterium was successfully immobilized with 10 % polyvinyl alcohol (PVA) and 1% sodium alginate (SA) and tannery effluent treatment were carried out using both free and immobilized cells of O. intermedium . Preliminary chemical oxygen demand (COD) reduction studies demonstrated 85 % and 54 % COD reductions by immobilized and free cells, respectively as compared to blank PVA beads (10 % COD). Both free and immobilized cells were able to degrade tannery effluent after 48 h but the reduction of total dissolved solids-TDS (56 %), COD (85 %), biochemical oxygen demand-BOD (68 %), and total chromium-TC (95 %) were higher with immobilized cells. The reduction of pollutants in tannery effluent was established by GC–MS analysis in which degradation of organic pollutants after treating with free and immobilized cells was observed. Phytotoxicity investigation of tannery effluents on Vigna radiata L seed showed that bacterial treatment not only allowed seeds germination but also supported seedling growth. The results from this flask-scale work suggest that immobilized cell methods of bacterial treatment can potentially be applied in tannery effluent treatment to reduce the pollution load as well as phytotoxic effects.