A New Exon Derived from a Mammalian Apparent LTR Retrotransposon of the SUPT16H Gene. (10th March 2013)

• Record Type: Journal Article
• Authors: Bae, Min-In; Kim, Yun-Ji; Lee, Ja-Rang; Jung, Yi-Deun; Kim, Heui-Soo
• Other Names: Gutierrez Soraya E. Academic Editor.
• Is Part Of: International journal of genomics. Volume 2013(2013)
• Publication Date: 2013-03-10
• Subjects: Genomes -- Periodicals; Genomics -- Periodicals; Cytogenetics -- Periodicals; Genomics; Genome; Molecular Biology; Cytogenetics; Genomes; Genomics; Periodicals; 572.86
• DOI: 10.1155/2013/387594
• Languages: English
• ISSNs: 2314-436X

Abstract

Abstract : The SUPT16H gene known as FACTP140 is required for the transcription of other genes. For transcription, genes need to be complexed with accessory factors, including transcription factors and RNA polymerase II. One such factor, FACT, interacts with histones H2A/H2B for nucleosome disassembly and transcription elongation. The SUPT16H gene has a transcript and many expressed sequence tags (ESTs). We were especially interested in an MaLR-derived transcript (EST, BX333035) that included a new exon introduced by a transposable element, a mammalian apparent LTR retrotransposon (MaLR). The MaLR was detected ranging from humans to galagos, indicating the MaLR in the SUPT16H gene is integrated into the primate ancestor genome. A new exon was created by alternative donor site provided by the MaLR. The original transcript and the MaLR-derived transcript were expressed in various human, rhesus monkey, and other primate tissues. Additionally, we identified a new alternative transcript that included the MaLR, but there was no significant difference in the expression of the original transcript and the MaLR-derived transcript. Interestingly, the new alternative transcript and the MaLR-derived transcript had the MaLR sequence in the new exon, but they had different structures by adopting different 3 ′ splice sites. From this study, we verified transposable elements that contributed to transcriptome diversity.