Simultaneous nitrogen and phosphorus removal from simulated digested piggery wastewater in a single-stage biofilm process coupling anammox and intracellular carbon metabolism. (August 2021)

• Record Type: Journal Article
• Authors: Huang, Wei; Zhou, Jian; He, Xuejie; He, Lei; Lin, Ziyuan; Shi, Shuohui; Zhou, Jiong
• Is Part Of: Bioresource technology. Volume 333(2021)
• Publication Date: 2021-08
• Subjects: Anammox -- Intracellular carbon -- Low C/N -- Digested piggery wastewater -- Nutrients removal -- Metagenomics; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125152
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: N and P could be efficiently removed with extremely low C/N in SAIC process. Intracellular carbon provided electrons for BPR and denitrification. Anammox contributed 67.40% of TN elimination at least. Anammox bacteria, nitrifiers, GAOs and PAOs coexisted in this system. Multiple metabolic pathways and their synergistic effect were revealed. Abstract: A Single-stage biofilm process coupling Anammox and Intracellular Carbon metabolism (SAIC) was constructed for treating simulated digested piggery wastewater with low carbon/nitrogen ratio (C/N) in this study. TN removal in SAIC system increased by more than 12.77% compared to the reference, and the maximum total phosphorus (TP) removal efficiency reached to 83.70% (C/N = 1.5). Denitrification driven by intracellular carbon, mainly poly-β-hydroxybutyrate (PHB, 78.57%), contributed 32.60% of TN elimination at most, and at least 67.40% should be attributed to anammox. Phosphorus was thought to be mainly removed through biological route, while chemical precipitation also explained around 10% of removed TP. Furthermore, commensalism of glycogen accumulating organisms (GAOs), phosphate accumulating organisms (PAOs), nitrifiers and anammox bacteria was revealed by combining 16S rRNA amplicon sequencing and metagenomics. As a result, multiple metabolic pathways including anammox, (partial) nitrification, endogenous (partial) denitrification and biological P-removal played synergistic effect in SAIC system.