Luffa operculata at a late period of gestation dysregulates melatonin and cytokines interfering with weight of dams and their male offspring. (15th July 2021)

• Record Type: Journal Article
• Authors: Alves, Cinthia dos S.; Frias, Humberto V.; Bonamin, Leoni V.; Correia, Michelle S.F.; Corrêa, Monica G.; Bondan, Eduardo F.; de Fátima M. Martins, Maria; Coelho, Cidéli P.; Bernardi, Maria M.; Suffredini, Ivana B.
• Is Part Of: Journal of ethnopharmacology. Volume 275(2021)
• Publication Date: 2021-07-15
• Subjects: Animal behavior -- Buchinha-do-norte -- Cognitive performance -- Interleukines -- Reproduction -- Vertical treatment; EBN buchinha-do-norte aqueous extract -- ECAU Ethics Committee for Animal Use -- GD gestation day -- PND post-natal day -- CG control group -- EG experimental group -- OF open field -- LDB light-dark box -- ACTH adrenocorticotropic hormone -- IL interleukine -- AST aspartate transaminase -- ALT alanine transaminase -- TNF tumor necrosis factor; Reproductive pharmacology -- Traditional medicine Meso- and Southern America; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2021.113867
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: The tea made with the fruits of Luffa operculata (L.) Cogn. (Cucurbitaceae; EBN) is popularly used as abortive. Aim of the study: The present work aimed at accessing how the exposition of female Wistar rats to 1.0 mg/kg of EBN (experimental group, EG), or distilled water (control group, CG), by gavage, at gestational days (GD) 17–21 interfered with the reproductive performance, and with dams' behavior after weaning. Materials and methods: At post-natal day 2 (PND2), the number of male and female pups was evaluated, as well as their weight. After weaning (PND21), dams were euthanized, and their liver and kidneys were removed for histological and biochemical analyses, while the blood was used in the evaluation of cytokines IL-1α, IL-1β, IL-6 and TNF-α, corticosterone, adrenocorticotrophic hormone, melatonin, AST, ALT and creatinine levels. Results and Discussion: Dams that were treated with EBN showed an anxiety-like behavior, weight loss at the end of gestation and weight gain at weaning, accompanied with a significant decrease in pro-inflammatory cytokines and in the melatonin level. No significant histological or biochemical alterations have occurred in the liver or kidneys. The number of female pups was significantly higher in the EG. The male pups showed weight gain at PND60. Conclusion: The presence of cucurbitacins is probably involved in the dysregulations that were found, due to their polycyclic steroid triterpene structure. Graphical abstract: Image 1 Highlights: EBN has diminished melatonin serum levels in female rats at PND21. EBN has diminished female rats' weight at GD21 and augmented at PND21. EBN vertical administration augmented male pups weight gain at PND60.