Molecular detection of carbapenem resistance genes in rectal swabs from patients in Gulf Cooperation Council hospitals. (June 2021)

• Record Type: Journal Article
• Authors: Alqahtani, M.; Tickler, I.A.; Al Deesi, Z.; AlFouzan, W.; Al Jabri, A.; Al Jindan, R.; Al Johani, S.; Alkahtani, S.A.; Al Kharusi, A.; Mokaddas, E.; Nabi, A.; Saeed, N.; Madian, A.; Whitmore, J.; Tenover, F.C.
• Is Part Of: Journal of hospital infection. Volume 112(2021)
• Page Start: 96
• Page End: 103
• Publication Date: 2021-06
• Subjects: Carbapenem resistance -- Carbapenemase genes -- Surveillance -- Gulf Cooperation Council -- OXA-48 -- CRE; Cross infection -- Periodicals; Cross infection -- Prevention -- Periodicals; Nosocomial infections -- Periodicals; Nosocomial infections -- Prevention -- Periodicals; Cross Infection -- Periodicals; Cross Infection -- prevention & control -- Periodicals; Infection Control -- Periodicals; Electronic journals; 614.44
• DOI: 10.1016/j.jhin.2021.03.027
• Languages: English
• ISSNs: 0195-6701

Abstract

Summary: Background: Gram-negative organisms harbouring carbapenem resistance genes (CRGs) are spreading globally, including in Gulf Cooperation Council (GCC) countries. However, relatively few data are available about carriage of CRGs in hospitalized patients in this region. Aim: To determine prevalence of CRG carriage and risk factors for colonization among patients in GCC hospitals. Methods: Rectal swabs were obtained from ∼50 intensive care unit (ICU) patients from each of 11 hospitals in five GCC countries between March and November 2019. The swabs were tested for the presence of bla KPC, bla NDM, bla VIM, bla IMP, and bla OXA-48 CRG using a commercial polymerase chain reaction test. Data on risk factors for colonization were collected and analysed. Findings: Of 529 specimens screened, 138 (26.1%) were positive for one or more CRGs. The positivity rates among the hospitals ranged from 8.0% to 67.3%; ∼20% of the positive specimens harboured ≥2 CRGs. The most common CRG detected was bla OXA-48, which was present in 82 specimens (15.5%). Additional CRGs included bla NDM, blaVIM, blaKPC, and blaIMP either alone or in combination. Overall, 31.1% of patients on antibiotics on admission to the ICU were positive for CRGs compared to 16.5% not on antibiotic therapy ( P < 0.001). CRG detection was also more common among patients aged >65 years ( P = 0.027) and increased with hospital length of stay ( P = 0.025). Conclusion: The rate of CRGs detected in hospitalized patients in GCC countries varied considerably. Prior antibiotic exposure, increasing age, and prolonged length of stay were associated with CRG detection.