Unravelling the anthelmintic bioactives from Jasminum grandiflorum L. subsp. Floribundum adopting in vitro biological assessment. (15th July 2021)

• Record Type: Journal Article
• Authors: Hussein, Dorria; El-Shiekh, Riham A.; Saber, Fatema R.; Attia, Marwa M.; Mousa, Mohamed R.; Atta, Attia H.; Abdel-Sattar, Essam; Mouneir, Samar M.
• Is Part Of: Journal of ethnopharmacology. Volume 275(2021)
• Publication Date: 2021-07-15
• Subjects: Jasminum grandiflorum subsp. Floribundum -- Oleaceae -- Flavonoids -- Secoirridoids -- Anthelmintic -- Equine parasites nematodes; 13C-NMR carbon-13 nuclear magnetic resonance -- 1H-NMR proton nuclear magnetic resonance -- BuOH n-butanol -- CD3OD Deuterated methanol -- H2O water -- MeOH Methanol -- SEM scanning electron microscope; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2021.114083
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Jasminum grandiflorum L. is a medicinal plant widely used in the traditional system of Medicine as an anthelmintic in ringworm infections, for treating ulcers, stomatitis, skin diseases, and wounds. Aim of the study: The emergence of resistance by different parasites to currently used chemicals has been reported. There are increasing needs for more effective and safer parasiticides. Therefore, the current study was designed to investigate the methanolic extract of the aerial parts of J. grandiflorum subsp . Floribundum (JGTE) to confirm its traditional uses as anthelmintic through a bioassay-guided fractionation and isolation of the active components with anthelmintic activity. Materials and methods: The JGTE was partitioned into dichloromethane (DCM-F) and n -butanol (BuOH-F) fractions. The JGTE, fractions, and the isolated compounds were tested in vitro for their anthelmintic activity using two nematodes; one larval stage of cestode and one arthropod. Four major compounds were isolated from the most active fraction (BuOH-F) including two flavonoids and two secoirridoid glycosides, identified as kaempferol-3- O -neohesperoside (1 ), rutin (2 ), oleuropein (3 ), and ligstroside (4 ). Results: Among the isolated compounds from most active fraction (BuOH-F), rutin (2 ) displayed the highest anthelmintic activity in a dose-dependent activity with IC50 of 41.04 μg/mL against H. muscae adult worm, followed by ligstroside (4 ) with IC50 of 50.56 μg/mL. Conclusions: These findings could advocate the traditional use of J. grandiflorum L. and provide further insight into the anthelmintic activity of flavonoids. Graphical abstract: Image 1 Highlights: This study rationalized the traditional use of J. grandiflorum L. as anthelmintic plant. Four major metabolites were isolated; kaempherol neohesperoside, rutin, oleuropein, and ligstroside. A bioassay-guided fractionation of J. grandiflorum extract revealed that flavonoids were the active metabolites. Rutin displayed the highest activity against Habronema muscae adult worm.