Bisphenol F and bisphenol S promote lipid accumulation and adipogenesis in human adipose-derived stem cells. (June 2021)

• Record Type: Journal Article
• Authors: Reina-Pérez, Iris; Olivas-Martínez, Alicia; Mustieles, Vicente; Ruiz-Ojeda, Francisco Javier; Molina-Molina, José Manuel; Olea, Nicolás; Fernández, Mariana F.
• Is Part Of: Food and chemical toxicology. Volume 152(2021)
• Publication Date: 2021-06
• Subjects: Endocrine disruptors -- Adipogenesis -- Bisphenol F (BPF) -- Bisphenol S (BPS) -- Obesity -- Bisphenol A (BPA); ACTB β-actin -- advanced-DMEM Advance-Dulbecco Modified Eagle Medium -- BPA bisphenol A -- BPF bisphenol F 4, 4′-methylenediphenol -- BPS bisphenol S 4, 4′-sulfonyldiphenol -- C/EBPα CCAT/enhancer-binding protein -- DEX dexamethasone -- DM differentiation medium -- DMSO dimethyl sulfoxide -- EDC endocrine disrupting chemical -- FABP4 fatty acid binding protein 4 -- FBS fetal bovine serum -- GM growth medium -- hASCs human adipose-derived stem cells -- INS human insulin -- HPRT1 hypoxanthine-guanine phosphoribosyltransferase-1 -- IBMX 3-isobutyl-1-methylxanthine -- LPL lipoprotein-lipase -- μg micrograms -- μM micromolar -- mM millimolar -- ml millilitre -- min minutes -- ng nanograms -- nm nanometers -- C- negative control -- ORO Oil Red O -- PPARγ peroxisome proliferator-activated receptor gamma -- PBS phosphate buffered saline -- C+ positive control -- qRT-PCR quantitative real-time polymerase chain reaction -- ROSI rosiglitazone -- SEM standard error of the mean -- TBS tris-buffered saline; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2021.112216
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Bisphenol F (BPF) and bisphenol S (BPS) are increasingly used as substitutes for bisphenol A (BPA), an endocrine disrupting chemical (EDC) with obesogenic activity. We investigated the in vitro effects of BPS and BPF on the adipogenesis of human adipose-derived stem cells (hASCs) exposed to different doses (0.01, 0.1, 1, 10 and 25 μM), stopping the adipogenic process at 7 or 14 days. Intracellular lipid accumulation was quantified by the Oil Red O assay, gene expression of peroxisome proliferator-activated receptor gamma ( PPARγ ), CCAT/enhancer-binding protein ( C/EBPα ), lipoprotein-lipase ( LPL ) and fatty acid binding protein 4 ( FABP4 ), by quantitative real-time polymerase chain reaction (qRT-PCR) and protein levels by Western Blot. hASCs with BPF or BPS produced a linear dose-response increase in intracellular lipid accumulation and in gene expression of the adipogenic markers, confirmed by protein levels. Co-treatment ICI 182, 780 significantly inhibited BPF- but not BPS-induced lipid accumulation. Given the affinity of bisphenols for diverse nuclear receptors, their obesogenic effects may result from a combination of pathways rather than a single mechanism. Further research is warranted on the manner in which chemicals interfere with adipogenic differentiation. To our best knowledge, this report shows for the first time the obesogenic potential of BPF in hASCs. Highlights: BPF and BPS promote intracellular lipid accumulation in hASCs. Expression of key adipogenic genes increases in response to BPF and BPS. Our findings suggest that BPF and BPS are not safe alternatives to BPA. The obesogenic potential of BPF in human adipogenesis is reported for the first time.